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农业重金属和新型污染物检测取得突破

重金属、农药、化肥以及不断出现的新型污染物,侵蚀着我国农业资源环境。2012年,国家863计划启动了“农业生境检测与修复技术研究”项目,由西北农林科技大学牵头,目前,已在农业生境中重金属和新型等污染物检测技术上取得突破性进展。
该项目开发出无固定化点靶标的核酸适配体筛选技术与信号表达技术,结合分子探和无固定化点靶标SELEX技术,开发出基于纳米金粒子聚集的共振散射信号表达技术等。该方法快速、灵敏度高、选择性好、操作简便。对铅离子最低检测限远低于美国EPA和WHO对饮用水中铅最高含量标准,对汞离子、四环素、三价砷的最低检测限均低于美国标准,检测限低于国际食品最高标准检测浓度的2—50倍。相关成果发表在相关领域国际著名期刊上,被英国皇家化学学会关注,获得高度评价,目前已发表SCI论文14篇,申报发明专利11项。
结合纳米金烧制、金标抗体制备、试纸条组装以及免疫试纸条检测技术,项目组研制出快速检测各种农药的免疫金标试纸条产品,建立了吡虫啉、甲基毒死蜱、杀螟硫磷、水胺硫磷四个单通道农药金标试纸条检测体系,检测时间为5分钟。
国际农药免疫检测成本高、耗时长,课题组首次以抗吡虫啉、水胺硫磷、甲基毒死蜱的三种高效单克隆抗体为基础,结合胶体金免疫层析试纸条技术,研发出简易型农药三通道半定量免疫快速检测试纸条,检测时间为7分钟,在青菜、水和土壤的样品检测中,回收率大于88%。

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